%0 Journal Article
%T Rapid Extraction of MtDNA from Cryptococcus neoformans and Examined by Electronic Microscopy<br>新生隐球菌线粒体的快速抽提和电镜观察 Rapid Extraction of MtDNA from Cryptococcus neoformans and Examined by Electronic Microscopy
%A ZOU Xian-biao
%A LIAO Wan-qing
%A WEN Hai
%A WU Jian-hua
%A QIU Yun
%A YANG Jin-shui
%A <br>邹先彪
%A 廖万清
%A 温海
%A 吴建华
%A 仇芸
%A 杨金水
%J 遗传
%D 2002
%I 
%X Cryptococcus neoformans may be grown to the exponential phase,are broken by a combination of NovoZym234 and mechanical means,and mitochondrial DNA was extracted from DNaseI-treated mitrochondrial preparetion by differential centrifugation.Three pellets,including yeast cell,protoplasts,mitochondrial,were examined by transmission electronic microscopy.The resulting mtDNA is sufficicently pure for restriction endonucleases analysis and PCR in further studying.A rapid and effective method for the preparation of the mtDNA of C.neoformans was established.
%K Cryptococcus neoformans
%K mitochondria
%K DNA
%K ultrastructure<br>新生隐球菌
%K 线粒体DNA
%K 超微结构
%K 快速抽提
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=B3CBF86C05312DF4&yid=C3ACC247184A22C1&vid=B91E8C6D6FE990DB&iid=CA4FD0336C81A37A&sid=BFE7933E5EEA150D&eid=95D537AC89B28832&journal_id=0253-9772&journal_name=遗传&referenced_num=0&reference_num=6