Cloning and sequence analysis of alpha satellite DNA in arctic fox
蓝狐-卫星DNA的克隆及序列分析

Genomic DNAs from Arctic fox were cleaved with restriction enzymes.The prominent ethidium-bromide stanined bands were cloned and sequenced after separation of DNA fragments by agarose gel electrophoresis.Forty-two satellite sequences were obtained by sequencing.Sequence analyses revealed that the satellite monomer in length was 737 bp,with an average G C content of 51.9%.There were 91%-97% homology among the monomers,and the monomer con-sisted of three subrepeats of approximately 245 bp in length with 51%-54% homology.The satellite DNAs were specific for Canidae,and had 74% homology to the dog centromere-associated nucleotide sequences.In order to distinguish from another satellite DNAs of canidae genome,the satellite was designated as alpha satellite DNA.