%0 Journal Article
%T Cloning and sequence analysis of alpha satellite DNA in arctic fox
蓝狐-卫星DNA的克隆及序列分析
%A YAN Shou-Qing
%A ZHU Wan-Ju
%A ZHANG Xue-Mei
%A LI Bing
%A SUN Jin-Hai
%A
闫守庆
%A 祝万菊
%A 张雪梅
%A 李冰
%A 孙金海
%J 遗传
%D 2007
%I
%X Genomic DNAs from Arctic fox were cleaved with restriction enzymes.The prominent ethidium-bromide stanined bands were cloned and sequenced after separation of DNA fragments by agarose gel electrophoresis.Forty-two satellite sequences were obtained by sequencing.Sequence analyses revealed that the satellite monomer in length was 737 bp,with an average G C content of 51.9%.There were 91%-97% homology among the monomers,and the monomer con-sisted of three subrepeats of approximately 245 bp in length with 51%-54% homology.The satellite DNAs were specific for Canidae,and had 74% homology to the dog centromere-associated nucleotide sequences.In order to distinguish from another satellite DNAs of canidae genome,the satellite was designated as alpha satellite DNA.
%K Canidae
%K arctic fox
%K satellite DNA
犬科动物
%K 蓝狐
%K 卫星DNA
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=870726A0C979F3182DE8613AF97F70EE&yid=A732AF04DDA03BB3&vid=771469D9D58C34FF&iid=59906B3B2830C2C5&sid=C825622C5A891845&eid=D80AE3757B45ED33&journal_id=0253-9772&journal_name=遗传&referenced_num=0&reference_num=12