The Polymorphism of Nine STR Loci on the Genetic Original Identification of Complete Hydatidiform Mole
9个多态性STR基因座用于完全性葡萄胎的亲代来源鉴定 The Polymorphism of Nine STR Loci on the Genetic Original Identification of Complete Hydatidiform Mole

To explore the genetic origin of hydatidiform mole (CHM),33 cases of CHM were collected mainly from Harbin Red Cross Central hospital from 1998.6 to 2001.5 and studied by multiplex-PCR,products were separated using denaturing polyacrylamide gel and were detected by silver stain for 9 different STR loci analysis. Among 33 samples of CHM,DNA from only paternal origin was found in 27 cases (81.8%,27/33),and from both parents in 6 cases (18.2%,6/33); and in the former,the homozygous CHM and the heterozygous CHM were 22 cases (66.7%,22/33) and 5 cases (15.1%,5/33),respectively. There was difference between analysis of microsatellite DNA polymorphism and pathological diagnosis in hydatidiform moles' classification. The results suggest that the analysis of 9 polymorphic STR loci is suitable for genetic original identification of hydatidiform moles.