The Problems and Solution Methods in Establishing and Maintaining ES Cell Lines
小鼠胚胎干细胞（ES细胞）建系和维持过程中的问题及对策 The Problems and Solution Methods in Establishing and Maintaining ES Cell Lines

Based on manipulating Embryonic Stem cells for more than ten years, we proposed our methods to solve the problems in establishing and maintaining ES cell lines, especially the improvement of routine operational approach.We also discussed some ambiguous problems and gave corresponding opinion and suggestion which was proved to be feasible and effective in our own research.