%0 Journal Article
%T The Problems and Solution Methods in Establishing and Maintaining ES Cell Lines<br>小鼠胚胎干细胞（ES细胞）建系和维持过程中的问题及对策 The Problems and Solution Methods in Establishing and Maintaining ES Cell Lines
%A MENG Guo liang
%A TANG Fu chou
%A TENG Lu
%A SHANG Ke gang
%A <br>孟国良
%A 汤富酬
%A 滕路
%A 尚克刚MENG Guo-liang
%A TANG Fu-chou
%A TENG Lu
%A SHANG Ke-gang
%J 遗传
%D 2001
%I 
%X Based on manipulating Embryonic Stem cells for more than ten years, we proposed our methods to solve the problems in establishing and maintaining ES cell lines, especially the improvement of routine operational approach.We also discussed some ambiguous problems and gave corresponding opinion and suggestion which was proved to be feasible and effective in our own research.
%K ES cell
%K Inner cell mass
%K pluripotency
%K normal diploid karyotype<br>ES细胞
%K 内细胞团
%K 多方向分化潜能
%K 正常二倍体核型
%K 建系
%K 培养
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=844D103C0C0F71D9&yid=14E7EF987E4155E6&vid=EA389574707BDED3&iid=38B194292C032A66&sid=8BB50A069C48D50B&eid=E39A3F4E3A67639B&journal_id=0253-9772&journal_name=遗传&referenced_num=15&reference_num=19