A Simple and Effective Method for Extracting Total RNA from Domestic Fungus
一种简单高效的食用真菌总RNA提取方法 A Simple and Effective Method for Extracting Total RNA from Domestic Fungus

With Flammulina velutipes material,an improved method was developed for extracting total RNA from domestic fungus that are rich in RNase,polyphenols,polymeric carbohydrates and proteoglycans..Phenol-chloroform-isoamyl alcohol were used twice to clear DNA and protein under higher concentration of denaturing solution and isopentanol,sodium acetate were used to precipitate RNA selectively.Pure and intact RNA can be effectively prepared by this method.