%0 Journal Article
%T A Simple and Effective Method for Extracting Total RNA from Domestic Fungus<br>一种简单高效的食用真菌总RNA提取方法 A Simple and Effective Method for Extracting Total RNA from Domestic Fungus
%A Kai-Song Zhou
%A Jiu-Gang Xue
%A Ning Chang
%A Chang Chen
%A Han-Xing Zhang
%A Chang-Kai Zhang
%A <br>周凯松
%A 薛久刚
%A 常宁
%A 陈畅
%A 张晗星
%A 张长铠ZHOU Kai-Song
%A XUE Jiu-Gang
%A CHANG Ning
%A CHEN Chang
%A ZHANG Han-Xing
%A ZHANG Chang-Kai
%J 遗传
%D 2003
%I 
%X With Flammulina velutipes material,an improved method was developed for extracting total RNA from domestic fungus that are rich in RNase,polyphenols,polymeric carbohydrates and proteoglycans..Phenol-chloroform-isoamyl alcohol were used twice to clear DNA and protein under higher concentration of denaturing solution and isopentanol,sodium acetate were used to precipitate RNA selectively.Pure and intact RNA can be effectively prepared by this method.
%K domestic fungus
%K RNA extracting
%K Flammulia velutipes<br>食用菌
%K RNA提取
%K 金针菇
%K 提取方法
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=767972BE8DA97114&yid=D43C4A19B2EE3C0A&vid=C5154311167311FE&iid=B31275AF3241DB2D&sid=7D2B339649A57040&eid=F20A770D14436F7C&journal_id=0253-9772&journal_name=遗传&referenced_num=2&reference_num=5