Rapid Amplification of 3′cDNA End of Suaeda liaotungensis Betaine Aldehyde Dehydrogenase Using One-step PCR
一步PCR快速扩增辽宁碱蓬甜菜碱醛脱氢酶cDNA 3′末端序列 Rapid Amplification of 3′cDNA End of Suaeda liaotungensis Betaine Aldehyde Dehydrogenase Using One-step PCR

Based on part of a known cDNA sequence of Suaeda liaotungensis betaine aldehyde dehydrogenase, we successfully cloned the 3' cDNA end of S. lianotungensis betaine aldehyde dehydrogenase using one-step PCR with a gene-specific primer and universal primer. Compared with the typical 3' RACE, one-step PCR is rapid, simple and inexpensive. It is very rapid to amplify an unknown cDNA 3' end using this method.