%0 Journal Article
%T Rapid Amplification of 3′cDNA End of Suaeda liaotungensis Betaine Aldehyde Dehydrogenase Using One-step PCR<br>一步PCR快速扩增辽宁碱蓬甜菜碱醛脱氢酶cDNA 3′末端序列 Rapid Amplification of 3′cDNA End of Suaeda liaotungensis Betaine Aldehyde Dehydrogenase Using One-step PCR
%A LI Qiu-li
%A GAO Xiao-rong
%A YUAN Xiao-dong
%A LIU Da-wei
%A AN Li-jia
%A <br>李秋莉
%A 高晓蓉
%A 袁晓东
%A 刘大伟
%A 安利佳LI Qiu-li
%A GAO Xiao-rong
%A YUAN Xiao-dong
%A LIU Da-wei
%A AN Li-jia
%J 遗传
%D 2002
%I 
%X Based on part of a known cDNA sequence of Suaeda liaotungensis betaine aldehyde dehydrogenase, we successfully cloned the 3' cDNA end of S. lianotungensis betaine aldehyde dehydrogenase using one-step PCR with a gene-specific primer and universal primer. Compared with the typical 3' RACE, one-step PCR is rapid, simple and inexpensive. It is very rapid to amplify an unknown cDNA 3' end using this method.
%K one-step PCR
%K Suaeda lianotungenesis
%K betaine aldehyde dehydrogenase<br>一步PCR
%K 辽宁碱蓬
%K 甜菜碱醛脱氢酶
%K 3′cDNA末端
%K 快速扩增
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=6265ECAD74FA87F1&yid=C3ACC247184A22C1&vid=B91E8C6D6FE990DB&iid=0B39A22176CE99FB&sid=E114CF9BB47B65BE&eid=7EBE588F611589FC&journal_id=0253-9772&journal_name=遗传&referenced_num=8&reference_num=4