Transfer of Antibacterial Peptide D Gene into Tomato and Identification of Transgenic Plants
抗菌肽D基因导入番茄及转基因植株的鉴定

By using pollen tube pathway,antibacterial peptide D(AP D) gene developed from Chinese oak silkwarm was transferred into tomato.PCR,dot and Southern blot analysis confirmed that stable integration of AP D gene into the tomato genome had occurred.The expression of NOP gene which was localized at downstream of AP D gene was confirmed with nopaline detection.In comparision with non transgenicplants,some transgenic plant expressed a stronger resistance value to bacterialwilt disease (Pseudomonas solanacearum).