%0 Journal Article
%T Transfer of Antibacterial Peptide D Gene into Tomato and Identification of Transgenic Plants
抗菌肽D基因导入番茄及转基因植株的鉴定
%A TIAN Chang-en
%A WANG Zheng-xun
%A CHEN Tao
%A ZHOU Yu-ping
%A HANG Zi-ran
%A HANG Ya-dong
%A
田长恩
%A 王正询
%A 陈韬
%A 周玉萍
%A 黄自然
%A 黄亚东
%J 遗传
%D 2000
%I
%X By using pollen tube pathway,antibacterial peptide D(AP D) gene developed from Chinese oak silkwarm was transferred into tomato.PCR,dot and Southern blot analysis confirmed that stable integration of AP D gene into the tomato genome had occurred.The expression of NOP gene which was localized at downstream of AP D gene was confirmed with nopaline detection.In comparision with non transgenicplants,some transgenic plant expressed a stronger resistance value to bacterialwilt disease (Pseudomonas solanacearum).
%K antibacterial peptide
%K D gene
%K pollen
%K tube pathway
%K tomato
%K bacterialwilt
抗菌肽D基因
%K 花粉通道法
%K 番茄
%K 青枯病
%K 抗病育种
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=AF45D89430678990C83DC9FE09B806FE&yid=9806D0D4EAA9BED3&vid=BC12EA701C895178&iid=0B39A22176CE99FB&sid=7AA74D31F1FF2DCE&eid=CFAC5CB624A41AFD&journal_id=0253-9772&journal_name=遗传&referenced_num=40&reference_num=17