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遗传 2002
A Template DNA Preparation Method for PCR and RAPD Reaction by Using Alkali-Treated Wheat Leaf Tissues
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Abstract:
In this paper we introduce a new method of PCR and RAPD reaction by using a small quantity of wheat leaf tissue with alkali treatment.It can be used either to detect target gene or to analyze polymorphism of wheat varieties.This method is simple and reliable,especially for a large-scale detection.