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ISSN: 2333-9721
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遗传  2002 

A Template DNA Preparation Method for PCR and RAPD Reaction by Using Alkali-Treated Wheat Leaf Tissues
小麦叶片直接用于PCR和RAPD反应的方法 A Template DNA Preparation Method for PCR and RAPD Reaction by Using Alkali-Treated Wheat Leaf Tissues

Keywords: wheat,genomic DNA,molecular marker,PCR,RAPD
小麦叶片
,PCR,RAPD反应,基因组DNA,分子标记

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Abstract:

In this paper we introduce a new method of PCR and RAPD reaction by using a small quantity of wheat leaf tissue with alkali treatment.It can be used either to detect target gene or to analyze polymorphism of wheat varieties.This method is simple and reliable,especially for a large-scale detection.

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