%0 Journal Article
%T A Template DNA Preparation Method for PCR and RAPD Reaction by Using Alkali-Treated Wheat Leaf Tissues<br>小麦叶片直接用于PCR和RAPD反应的方法 A Template DNA Preparation Method for PCR and RAPD Reaction by Using Alkali-Treated Wheat Leaf Tissues
%A WANG Xiu feng
%A YANG Jian bo
%A WU Li fang
%A LI Li
%A XIANG Tai he
%A <br>汪秀峰
%A 杨剑波
%A 吴丽芳
%A 李莉
%A 向太和WANG Xiu-feng
%A YANG Jian-bo
%A WU Li-fang
%A LI Li
%A XIANG Tai-he
%J 遗传
%D 2002
%I 
%X In this paper we introduce a new method of PCR and RAPD reaction by using a small quantity of wheat leaf tissue with alkali treatment.It can be used either to detect target gene or to analyze polymorphism of wheat varieties.This method is simple and reliable,especially for a large-scale detection.
%K wheat
%K genomic DNA
%K molecular marker
%K PCR
%K RAPD<br>小麦叶片
%K PCR
%K RAPD反应
%K 基因组DNA
%K 分子标记
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=F0049894DC70B8B6&yid=C3ACC247184A22C1&vid=B91E8C6D6FE990DB&iid=38B194292C032A66&sid=2E01F39B6CBD53DE&eid=9DC563A0FEFC04F9&journal_id=0253-9772&journal_name=遗传&referenced_num=7&reference_num=5