Targeted Gene Correction Directed by Chimeric RNA/DNA Oligonucleotides
RNA/DNA嵌合分子介导的高效基因修复 Targeted Gene Correction Directed by Chimeric RNA/DNA Oligonucleotides

We introduce a new technique targeted gene correction directed by chimeric RNA/DNA oligonucleotides which began at 1996.It uses synthetic double stranded non circular RNA/DNA chimeric oligonucleotides to transfect cells and make a single based change at the targeted site of the target gene.It is highly efficient (the highest efficiency is more than 50%),highly special,safe,without danger of mutation caused by random insertion,without immune response,and without obvious toxicity.It can be used to make point mutation,or gene knock out plants and animals,and is very likely to be used in human gene therapy in the near future.It is also valuable in the study of functional genomics,pharmacogenetics,and medicine.