%0 Journal Article
%T Targeted Gene Correction Directed by Chimeric RNA/DNA Oligonucleotides<br>RNA/DNA嵌合分子介导的高效基因修复 Targeted Gene Correction Directed by Chimeric RNA/DNA Oligonucleotides
%A TANG Fu-chou
%A HAN Rong
%A XUE You-fang
%A <br>汤富酬
%A 韩嵘
%A 薛友纺TANG Fu-chou
%A HAN Rong
%A XUE You-fang
%J 遗传
%D 2000
%I 
%X We introduce a new technique targeted gene correction directed by chimeric RNA/DNA oligonucleotides which began at 1996.It uses synthetic double stranded non circular RNA/DNA chimeric oligonucleotides to transfect cells and make a single based change at the targeted site of the target gene.It is highly efficient (the highest efficiency is more than 50%),highly special,safe,without danger of mutation caused by random insertion,without immune response,and without obvious toxicity.It can be used to make point mutation,or gene knock out plants and animals,and is very likely to be used in human gene therapy in the near future.It is also valuable in the study of functional genomics,pharmacogenetics,and medicine.
%K RNA/DNA chimeric oligonucleotides
%K gene therapy
%K gene correction<br>RNA／DNA嵌合分子
%K 基因治疗
%K 基因修复
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=DBF37E97120E0B57&yid=9806D0D4EAA9BED3&vid=BC12EA701C895178&iid=E158A972A605785F&sid=FDC7AF55F77D8CD4&eid=DC330B09A33F1455&journal_id=0253-9772&journal_name=遗传&referenced_num=2&reference_num=6