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遗传 2003
Recombination and Fusion Expression of Porcine Defensin Gene PBD-I in E.coli
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Abstract:
The porcine defensin gene PBD-I was amplified by RT-PCR, then the gene was inserted into expression vector PinPoint(TM) Xa-3. Recombinant plasmid named as ppd-1 was transformed into E.Coli JM109, which could effectively produce fusion protein induced with IPTG. The positive clone of PBD-I gene expressed 17kDa fusion protein by SDS-PAGE electrophoresis. Expression of PBD-I gene didn't increase distinctly along with time. The expression of PBD-I gene lays a foundation in research on antimicrobial activities and its mechanism of the defensin.
