%0 Journal Article
%T Recombination and Fusion Expression of Porcine Defensin Gene PBD-I in E.coli<br>猪防御素基因PBD-I在大肠杆菌中的重组和融合表达
%A Gang Luo
%A Hong Wei
%A <br>罗刚
%A 魏泓
%J 遗传
%D 2003
%I 
%X The porcine defensin gene PBD-I was amplified by RT-PCR, then the gene was inserted into expression vector PinPoint(TM) Xa-3. Recombinant plasmid named as ppd-1 was transformed into E.Coli JM109, which could effectively produce fusion protein induced with IPTG. The positive clone of PBD-I gene expressed 17kDa fusion protein by SDS-PAGE electrophoresis. Expression of PBD-I gene didn't increase distinctly along with time. The expression of PBD-I gene lays a foundation in research on antimicrobial activities and its mechanism of the defensin.
%K porcine defensin
%K gene recombination
%K fusion<br>猪防御素
%K 基因重组
%K 融合
%K 抗菌肽
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=22CC6B0EAA232E38&yid=D43C4A19B2EE3C0A&vid=C5154311167311FE&iid=0B39A22176CE99FB&sid=A020552C37306588&eid=2DBBF45CC176713E&journal_id=0253-9772&journal_name=遗传&referenced_num=5&reference_num=9