Progress of the PCR Amplification Techniques for Chromosome Walking
应用于染色体步移的PCR扩增技术的研究进展

Various PCR-based methods are available for chromosome walking from a known sequence to an unknown region.It is promising in genome-related research for the following experiments: promoter cloning,obtaining non-conservative parts of genes in new species,identification of T-DNA or transposon insertion sites and filling in gaps or unknown chromosome regions in genome sequencing.These methods consisted of three types: inverse PCR,ligation-mediated PCR and specific primer PCR.In this review,we illustrated and compared the current techniques.