%0 Journal Article
%T Progress of the PCR Amplification Techniques for Chromosome Walking<br>应用于染色体步移的PCR扩增技术的研究进展
%A LIU Bo
%A SU Qiao
%A TANG Min-Qian
%A YUAN Xiao-Dong
%A AN Li-Jia
%A <br>刘博
%A 苏乔
%A 汤敏谦
%A 袁晓东
%A 安利佳
%J 遗传
%D 2006
%I 
%X Various PCR-based methods are available for chromosome walking from a known sequence to an unknown region.It is promising in genome-related research for the following experiments: promoter cloning,obtaining non-conservative parts of genes in new species,identification of T-DNA or transposon insertion sites and filling in gaps or unknown chromosome regions in genome sequencing.These methods consisted of three types: inverse PCR,ligation-mediated PCR and specific primer PCR.In this review,we illustrated and compared the current techniques.
%K chromosome walking
%K inverse PCR
%K ligation-mediated PCR
%K specific primer PCR<br>染色体步移
%K 反向PCR
%K 连接法PCR
%K 特异引物PCR
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=1F02AAEC6D6186C7&yid=37904DC365DD7266&vid=D3E34374A0D77D7F&iid=94C357A881DFC066&sid=D5970ECA7D10A7B1&eid=240CB58995465C01&journal_id=0253-9772&journal_name=遗传&referenced_num=13&reference_num=41