Identification for Genetically Modified Maize T14/T25 with Real Time Fluorenscent PCR Method
用实时荧光PCR方法鉴定转基因玉米T14/T25Identification for Genetically Modified Maize T14/T25 with Real Time Fluorenscent PCR Method

To identify genetically modified (GM) maize T14/T25 lines,a real-time fluorescent PCR (RTF PCR) assay was performed in this study. Primers and Taq man probes specific for inserted genes in the T14/T25 were used to conduct the real-time fluorenscent (RTF) PCR and PCR assays.The RTF PCR method was established to detect and identify GM maize lines.The results show that the Taq Man probe could identify T14/T25 maize used,while other GM and NO-GM maize didn't be detected.The RTF PCR could be a new method for detecting other genetically modified organism.