%0 Journal Article
%T Identification for Genetically Modified Maize T14/T25 with Real Time Fluorenscent PCR Method<br>用实时荧光PCR方法鉴定转基因玉米T14/T25Identification for Genetically Modified Maize T14/T25 with Real Time Fluorenscent PCR Method
%A CAO Ji-Juan~
%A <br>曹际娟
%A 覃 文
%A 朱水芳
%A 曹远银CAO Ji-Juan
%A QIN wen
%A ZHU Shui-Fang
%A CAO Yuan-Yin
%J 遗传
%D 2004
%I 
%X To identify genetically modified (GM) maize T14/T25 lines,a real-time fluorescent PCR (RTF PCR) assay was performed in this study. Primers and Taq man probes specific for inserted genes in the T14/T25 were used to conduct the real-time fluorenscent (RTF) PCR and PCR assays.The RTF PCR method was established to detect and identify GM maize lines.The results show that the Taq Man probe could identify T14/T25 maize used,while other GM and NO-GM maize didn't be detected.The RTF PCR could be a new method for detecting other genetically modified organism.
%K real-time fluorescent PCR
%K T14/T25 GM maize
%K identification<br>实时荧光PCR
%K T14/T25转基因玉米
%K 鉴定
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=409D6DA9708305FE&yid=D0E58B75BFD8E51C&vid=96C778EE049EE47D&iid=94C357A881DFC066&sid=128B7AEF80A42C95&eid=D02611D1F8166C9A&journal_id=0253-9772&journal_name=遗传&referenced_num=4&reference_num=14