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物理学报 2006
Time-resolved two-photon excitation fluorescence spectroscopy
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Abstract:
A novel time_resolved two_photon excitation fluorescence spectroscopic system based on a high repetition rate streak camera and capable of simultaneous measurement of fluorescence spectrum and lifetime of the samples is presented. The system has high temporal (6.5_200 ps) and spectral (1_3 nm) resolutions and permits rapid data acquisition and reliable and reproducible lifetime and spectrum determinations. The system is tested with standard fluorescent dyes (rhodamine 6G, coumarin 314 and their mixtures) and the lifetimes and spectra obtained agree well with those reported in the literatures. Experimental results indicate that our system provides a unique contrast mechanism for discriminating multi_fluorophore or multi_component of biological tissues through spectrally and temporally resolved information and may find extensive applications in multispectral fluorescence lifetime imaging and fluorescence resonance energy transfer imaging.
