%0 Journal Article
%T Time-resolved two-photon excitation fluorescence spectroscopy<br>双光子激发时间分辨荧光光谱测量技术
%A Liu Li-Xin
%A Qu Jun-Le
%A Lin Zi-Yang
%A Chen Dan-Ni
%A Xu Gai-Xi
%A Hu Tao
%A Guo Bao-Ping
%A Niu Han-Ben
%A <br>刘立新
%A 屈军乐
%A 林子扬
%A 陈丹妮
%A 许改霞
%A 胡 涛
%A 郭宝平
%A 牛憨笨
%J 物理学报
%D 2006
%I 
%X A novel time_resolved two_photon excitation fluorescence spectroscopic system based on a high repetition rate streak camera and capable of simultaneous measurement of fluorescence spectrum and lifetime of the samples is presented. The system has high temporal (6.5_200 ps) and spectral (1_3 nm) resolutions and permits rapid data acquisition and reliable and reproducible lifetime and spectrum determinations. The system is tested with standard fluorescent dyes (rhodamine 6G, coumarin 314 and their mixtures) and the lifetimes and spectra obtained agree well with those reported in the literatures. Experimental results indicate that our system provides a unique contrast mechanism for discriminating multi_fluorophore or multi_component of biological tissues through spectrally and temporally resolved information and may find extensive applications in multispectral fluorescence lifetime imaging and fluorescence resonance energy transfer imaging.
%K fluorescence lifetime
%K fluorescence spectrum
%K two-photon excitation
%K high repetition rate streak camera<br>荧光寿命
%K 荧光光谱
%K 双光子激发
%K 高重复频率扫描相机
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=47EA7CFDDEBB28E0&jid=29DF2CB55EF687E7EFA80DFD4B978260&aid=39F394B7A38B9897&yid=37904DC365DD7266&vid=E514EE58E0E50ECF&iid=59906B3B2830C2C5&sid=EE2B3B4485E38CC7&eid=8061A408883D8D3C&journal_id=1000-3290&journal_name=物理学报&referenced_num=0&reference_num=28