High expression and characterization of N-acyl-homoserine lactonase-xylanase recombinant protein
高效表达N-乙酰高丝氨酸内酯酶-木聚糖酶融合蛋白及其酶学性质

Objective] The fusion protein SL2B with both xylanase and N-acyl-homoserine lactonase activities was expressed in Pichia pastori.Characterization of the purified xylanase and N-acyl-homoserine lactonase fusion protein SL2B was investigated.Methods] The fusion gene sl2b was amplified from the N-acyl-homoserine lactonase gene aiiA-B546 and the xylanase gene xynAS27cd via overlap PCR technique.After the recombinant vector pPIC9/sl2b was transformed into P.pastoris,transformants with both xylanase and N-acyl-...