%0 Journal Article
%T High expression and characterization of N-acyl-homoserine lactonase-xylanase recombinant protein<br>高效表达N-乙酰高丝氨酸内酯酶-木聚糖酶融合蛋白及其酶学性质
%A Meichao Zhang
%A Yanan Cao
%A Bin Yao
%A Zhigang Zhou
%A <br>张美超
%A 曹雅男
%A 姚斌
%A 周志刚
%J 微生物学报
%D 2011
%I 
%X Objective] The fusion protein SL2B with both xylanase and N-acyl-homoserine lactonase activities was expressed in Pichia pastori.Characterization of the purified xylanase and N-acyl-homoserine lactonase fusion protein SL2B was investigated.Methods] The fusion gene sl2b was amplified from the N-acyl-homoserine lactonase gene aiiA-B546 and the xylanase gene xynAS27cd via overlap PCR technique.After the recombinant vector pPIC9/sl2b was transformed into P.pastoris,transformants with both xylanase and N-acyl-...
%K fusion expression
%K enzymatic properties
%K n-acyl-homoserine lactonase<br>关键词:
%K 高丝氨酸内酯酶，木聚糖酶，融合表达，酶学性质
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=EEC77E274DE7318A25D840374CF6136A&yid=9377ED8094509821&vid=987EDA49D8A7A635&iid=5D311CA918CA9A03&sid=33C2C2DAF31F219B&eid=29036368246435B2&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=18