Construction of BV-T7 hybrid expression system based on baculovirus to express target gene eGFP in mammalian and chicken cells
构建基于杆状病毒的BV-T7杂合表达体系及利用其在哺乳动物和禽类细胞中表达eGFP基因

Objective]To investigate whether the recombinant baculovirus BV-T7 hybrid expression system can be effectively transduced into chicken cells in vitro,and whether it can express the foreign genes(eGFP).Method] We established a hybrid baculovirus-T7 RNA polymerase system for transient expression in mammalian cells and chicken cells.Two recombinant baculoviruses were constructed,one carrying cDNA of bacteriophage T7 RNA polymerase,with a nuclear localization signal,under the control of a mammalian promoter and the other expressing eGFP gene under the control of T7 promoter.The constructed recombinant baculoviruses co-infected mammalian oligodendrocyte cells,as well as chicken embryo fibroblasts cells and chicken embryo skeletal muscle cells.Results] The eGFP activity was detected in mammalian cell lines and embryo fibroblasts cells and chicken embryo skeletal muscle cells.The recombinant baculovirus transduction efficiency of oligodendrocyte cells was 59.5%,and in CEF cells and myoblast cells the transduction efficiencies were 23.2% and 33.1%.Conclusion] BV-T7 hybrid expression could be expressed T7 RNAP in mammalian cells and avian cells.