%0 Journal Article
%T Construction of BV-T7 hybrid expression system based on baculovirus to express target gene eGFP in mammalian and chicken cells<br>构建基于杆状病毒的BV-T7杂合表达体系及利用其在哺乳动物和禽类细胞中表达eGFP基因
%A Jingping Ge
%A Xiaoyan Tang
%A Dongni Gao
%A Shanshan Song
%A Shan Lu
%A Zhuangwei Lou
%A Wenxiang Ping
%A <br>葛菁萍
%A 唐晓艳
%A 高冬妮
%A 宋姗姗
%A 鲁珊
%A 楼庄伟
%A 平文祥
%J 微生物学报
%D 2012
%I 
%X Objective]To investigate whether the recombinant baculovirus BV-T7 hybrid expression system can be effectively transduced into chicken cells in vitro,and whether it can express the foreign genes(eGFP).Method] We established a hybrid baculovirus-T7 RNA polymerase system for transient expression in mammalian cells and chicken cells.Two recombinant baculoviruses were constructed,one carrying cDNA of bacteriophage T7 RNA polymerase,with a nuclear localization signal,under the control of a mammalian promoter and the other expressing eGFP gene under the control of T7 promoter.The constructed recombinant baculoviruses co-infected mammalian oligodendrocyte cells,as well as chicken embryo fibroblasts cells and chicken embryo skeletal muscle cells.Results] The eGFP activity was detected in mammalian cell lines and embryo fibroblasts cells and chicken embryo skeletal muscle cells.The recombinant baculovirus transduction efficiency of oligodendrocyte cells was 59.5%,and in CEF cells and myoblast cells the transduction efficiencies were 23.2% and 33.1%.Conclusion] BV-T7 hybrid expression could be expressed T7 RNAP in mammalian cells and avian cells.
%K recombinant baculovirus
%K T7 RNA polymerase/T7 promoter
%K eGFP
%K OL cells
%K chike-embryo primary cells<br>重组杆状病毒
%K T7RNA聚合酶/启动子
%K eGFP
%K OL细胞
%K 鸡胚原代细胞
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=1DA214E6FAACB1340959A11D279B9651&yid=99E9153A83D4CB11&vid=286FB2D22CF8D013&iid=38B194292C032A66&sid=3C6F5C97A07587AE&eid=86C0C9A759FDA8CA&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=0