Development of a genetic modification system for caerulomycin producer Actinoalloteichus sp. WH1-2216-6
浅蓝霉素产生菌海洋异壁放线菌WH1-2216-6 遗传操作体系的建立

Objective] In order to enable the caerulomyicn biosynthetic study by in vivo gene disruptions,it is crucial to develop a genetic modification system for the producer Actinoalloteichus sp.WH1-2216-6.Methods] The spore germination timing and the concentration of MgSO4 in the medium were investigated for the optimal conjugal transfer of exotic pSET152 DNA into Actinoalloteichus sp.WH1-2216-6.Using the PCR-targeting system,we disrupted a putative caerulomycin 2,3-dihydroxybenzoate-AMP ligase gene by in-frame ...