%0 Journal Article
%T Development of a genetic modification system for caerulomycin producer Actinoalloteichus sp. WH1-2216-6<br>浅蓝霉素产生菌海洋异壁放线菌WH1-2216-6 遗传操作体系的建立
%A Qinheng Lin
%A Guangtao Zhang
%A Sumei Li
%A Haibo Zhang
%A Jianhua Ju
%A WeimingZhu
%A Changsheng Zhang
%A <br>林钦恒
%A 张光涛
%A 李苏梅
%A 张海波
%A 鞠建华
%A 朱伟明
%A 张长生
%J 微生物学报
%D 2011
%I 
%X Objective] In order to enable the caerulomyicn biosynthetic study by in vivo gene disruptions,it is crucial to develop a genetic modification system for the producer Actinoalloteichus sp.WH1-2216-6.Methods] The spore germination timing and the concentration of MgSO4 in the medium were investigated for the optimal conjugal transfer of exotic pSET152 DNA into Actinoalloteichus sp.WH1-2216-6.Using the PCR-targeting system,we disrupted a putative caerulomycin 2,3-dihydroxybenzoate-AMP ligase gene by in-frame ...
%K actinoalloteichus
%K genetic manipulation system
%K caerulomycin
%K biosynthesis
%K 2
%K 3-dihydroxybenzoate-AMP ligase<br>关键词:
%K 异壁放线菌，遗传体系，浅蓝霉素，生物合成，二羟基苯甲酸甲酯AMP
%K 连接酶
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=EEC77E274DE7318A03D139B6D571FB9C&yid=9377ED8094509821&vid=987EDA49D8A7A635&iid=5D311CA918CA9A03&sid=31CCC5D591A72A74&eid=8F39F7FDA07C2566&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=27