%0 Journal Article %T Development of a genetic modification system for caerulomycin producer Actinoalloteichus sp. WH1-2216-6
浅蓝霉素产生菌海洋异壁放线菌WH1-2216-6 遗传操作体系的建立 %A Qinheng Lin %A Guangtao Zhang %A Sumei Li %A Haibo Zhang %A Jianhua Ju %A WeimingZhu %A Changsheng Zhang %A
林钦恒 %A 张光涛 %A 李苏梅 %A 张海波 %A 鞠建华 %A 朱伟明 %A 张长生 %J 微生物学报 %D 2011 %I %X Objective] In order to enable the caerulomyicn biosynthetic study by in vivo gene disruptions,it is crucial to develop a genetic modification system for the producer Actinoalloteichus sp.WH1-2216-6.Methods] The spore germination timing and the concentration of MgSO4 in the medium were investigated for the optimal conjugal transfer of exotic pSET152 DNA into Actinoalloteichus sp.WH1-2216-6.Using the PCR-targeting system,we disrupted a putative caerulomycin 2,3-dihydroxybenzoate-AMP ligase gene by in-frame ... %K actinoalloteichus %K genetic manipulation system %K caerulomycin %K biosynthesis %K 2 %K 3-dihydroxybenzoate-AMP ligase
关键词: %K 异壁放线菌,遗传体系,浅蓝霉素,生物合成,二羟基苯甲酸甲酯AMP %K 连接酶 %U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=EEC77E274DE7318A03D139B6D571FB9C&yid=9377ED8094509821&vid=987EDA49D8A7A635&iid=5D311CA918CA9A03&sid=31CCC5D591A72A74&eid=8F39F7FDA07C2566&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=27