Quantity of Desulfovibrios and analysis of intestinal microbiota diversity in health and intestinal disease people in Wuxi,Jiangsu province
江苏无锡健康与肠病人群肠道脱硫弧菌数量及肠道菌群多样性

Objective]This paper provides an overview of Desulfovibrio(DSV) incidence and its effect on bacterial diversity in human gastrointestinal tract of four groups: ulcerative colitis(UC),colorectal cancer(CRC),polypus(PP) and the healthy control(H).Methods] Real time fluorescence quantitative PCR(RT-PCR) assays were used to enumerate DSV in gastrointestinal tract of 58 subjects.Diversity of gut microbiota was analyzed by PCR-Denaturing Gradient Gel Electrophoresis(PCR-DGGE) and 16S rRNA V3 sequencing.Results] RT-PCR detected DSV in all samples.Significantly increased numbers of DSV were observed for UC and PP groups compared with CRC and H groups.No significant difference was observed for CRC and H groups with gene copy numbers of DSV.Alterations of DSV and gut microbiota were observed in disease groups.Conclusion] We found that quantity and diversity of DSV are significantly increased in UC and PP compared to controls.The increased numbers of DSV in disease groups suggests a possible harmful role.