%0 Journal Article
%T Quantity of Desulfovibrios and analysis of intestinal microbiota diversity in health and intestinal disease people in Wuxi,Jiangsu province<br>江苏无锡健康与肠病人群肠道脱硫弧菌数量及肠道菌群多样性
%A Junrong Ding
%A Qiuxiang Zhang
%A Xiaoming Liu
%A Gang Wang
%A Fengwei Tian
%A Hao Zhang
%A Wei Chen
%A <br>丁俊荣
%A 张秋香
%A 刘小鸣
%A 王刚
%A 田丰伟
%A 张灏
%A 陈卫
%J 微生物学报
%D 2012
%I 
%X Objective]This paper provides an overview of Desulfovibrio(DSV) incidence and its effect on bacterial diversity in human gastrointestinal tract of four groups: ulcerative colitis(UC),colorectal cancer(CRC),polypus(PP) and the healthy control(H).Methods] Real time fluorescence quantitative PCR(RT-PCR) assays were used to enumerate DSV in gastrointestinal tract of 58 subjects.Diversity of gut microbiota was analyzed by PCR-Denaturing Gradient Gel Electrophoresis(PCR-DGGE) and 16S rRNA V3 sequencing.Results] RT-PCR detected DSV in all samples.Significantly increased numbers of DSV were observed for UC and PP groups compared with CRC and H groups.No significant difference was observed for CRC and H groups with gene copy numbers of DSV.Alterations of DSV and gut microbiota were observed in disease groups.Conclusion] We found that quantity and diversity of DSV are significantly increased in UC and PP compared to controls.The increased numbers of DSV in disease groups suggests a possible harmful role.
%K real time PCR
%K DGGE
%K Desulfovibrio
%K intestinal diseases
%K 16S rRNA
%K dsrB<br>荧光定量PCR
%K DGGE
%K 脱硫弧菌
%K 肠道疾病
%K 16S
%K rRNA
%K dsrB
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=1D086AF23E396C1E71ED87AC085BC64B&yid=99E9153A83D4CB11&vid=286FB2D22CF8D013&iid=5D311CA918CA9A03&sid=BE9F677535B03A98&eid=C7A7E1E32985D389&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=0