Construction of ectoine absorption defective mutant for efficient ectoine production
四氢嘧啶吸收缺陷突变株高效制备四氢嘧啶

Objective] To further enhance ectoine(1,4,5,6-tetrahydro-2-methyl-4-pyrimidinecarboxylic acid) synthesis efficiency.Methods] We cloned Halomonas salina DSM 5928T teaABC gene of ectoine-specific transporter TeaABC by walking PCR and constructed ectoine absorption defective mutant H.salina DSM 5928T(teaABC-) by Red recombination technology.Results] The total concentration of ectoine and productivity in a 10 L fermentor were 9.10(±0.08) g/L and 9.93(±0.09) g/L.d.Conclusion] The ectoine absorption defect mutant H.salina DSM 5928T(teaABC-) compromised the negative feedback regulation of ectoine synthesis,which can significantly improve the efficiency of ectoine production.