%0 Journal Article
%T Construction of ectoine absorption defective mutant for efficient ectoine production<br>四氢嘧啶吸收缺陷突变株高效制备四氢嘧啶
%A Rui Xu
%A Linghua Zhang
%A <br>徐蕊
%A 张苓花
%J 微生物学报
%D 2012
%I 
%X Objective] To further enhance ectoine(1,4,5,6-tetrahydro-2-methyl-4-pyrimidinecarboxylic acid) synthesis efficiency.Methods] We cloned Halomonas salina DSM 5928T teaABC gene of ectoine-specific transporter TeaABC by walking PCR and constructed ectoine absorption defective mutant H.salina DSM 5928T(teaABC-) by Red recombination technology.Results] The total concentration of ectoine and productivity in a 10 L fermentor were 9.10(±0.08) g/L and 9.93(±0.09) g/L.d.Conclusion] The ectoine absorption defect mutant H.salina DSM 5928T(teaABC-) compromised the negative feedback regulation of ectoine synthesis,which can significantly improve the efficiency of ectoine production.
%K H
%K salina DSM 5928T
%K ectoine
%K teaABC
%K Red recombination
%K absorption defective mutant<br>H.salina
%K DSM
%K 5928T
%K 四氢嘧啶
%K teaABC
%K Red重组
%K 吸收缺陷突变株
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=FDB09CD97DEFC4B674B417C8014F294C&yid=99E9153A83D4CB11&vid=286FB2D22CF8D013&iid=94C357A881DFC066&sid=8DDBA6455F2E3ECF&eid=06DAE5E1DF7D0B6A&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=0