High-throughput screening of human pancreatic alpha-amylase inhibitors
人胰腺α-淀粉酶抑制剂高通量筛选模型的建立及其应用

Objective] Targeting the important enzyme in human glucose metabolic pathway,we established a high throughput screening model for human pancreatic α-amylase inhibitors.Methods] Pichia pastoris expression system was used to clone and express the human pancreatic α-amylase;we established the α-amylase inhibitor screening model using the catalytic properties of enzyme;this model was applied in screening of actinomycete’ metabolites;the taxonomic status of positive strains were analyzed by constructing 16S rRNA phylogenetic tree.Results] We cloned and expressed the intact gene of human pancreatic α-amylase successfully;the high-throughput screening model of α-amylase inhibitors was established;nearly 2000 actinomycete’ metabolites were screened,14 α-amylase inhibitor producing strains were obtained finally,and showed taxonomically rich diversity.Conclusion] The α-amylase inhibitor high-throughput screening model had high practical value for developing new hypoglycemic drugs.