%0 Journal Article %T High-throughput screening of human pancreatic alpha-amylase inhibitors
人胰腺α-淀粉酶抑制剂高通量筛选模型的建立及其应用 %A Xizhen Qi %A Limei Ren %A Fang Zheng %A Qi Zhang %A Fang Bai %A Gang Bai %A
齐西珍 %A 任丽梅 %A 郑芳 %A 张奇 %A 白芳 %A 白钢 %J 微生物学报 %D 2011 %I %X Objective] Targeting the important enzyme in human glucose metabolic pathway,we established a high throughput screening model for human pancreatic α-amylase inhibitors.Methods] Pichia pastoris expression system was used to clone and express the human pancreatic α-amylase;we established the α-amylase inhibitor screening model using the catalytic properties of enzyme;this model was applied in screening of actinomycete’ metabolites;the taxonomic status of positive strains were analyzed by constructing 16S rRNA phylogenetic tree.Results] We cloned and expressed the intact gene of human pancreatic α-amylase successfully;the high-throughput screening model of α-amylase inhibitors was established;nearly 2000 actinomycete’ metabolites were screened,14 α-amylase inhibitor producing strains were obtained finally,and showed taxonomically rich diversity.Conclusion] The α-amylase inhibitor high-throughput screening model had high practical value for developing new hypoglycemic drugs. %K α-amylase inhibitor %K Pichia pastoris expression system %K human pancreatic α-amylase %K actinomycetes %K high-throughput screening
α-淀粉酶抑制剂 %K 毕赤酵母表达系统 %K 人胰腺α-淀粉酶 %K 放线菌 %K 高通量药筛 %U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=EEC77E274DE7318A38AABC03DEF631E2&yid=9377ED8094509821&vid=987EDA49D8A7A635&iid=5D311CA918CA9A03&sid=2D52C01434B71375&eid=1AA557EFF1C6B447&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=13