%0 Journal Article
%T High-throughput screening of human pancreatic alpha-amylase inhibitors<br>人胰腺α-淀粉酶抑制剂高通量筛选模型的建立及其应用
%A Xizhen Qi
%A Limei Ren
%A Fang Zheng
%A Qi Zhang
%A Fang Bai
%A Gang Bai
%A <br>齐西珍
%A 任丽梅
%A 郑芳
%A 张奇
%A 白芳
%A 白钢
%J 微生物学报
%D 2011
%I 
%X Objective] Targeting the important enzyme in human glucose metabolic pathway,we established a high throughput screening model for human pancreatic α-amylase inhibitors.Methods] Pichia pastoris expression system was used to clone and express the human pancreatic α-amylase;we established the α-amylase inhibitor screening model using the catalytic properties of enzyme;this model was applied in screening of actinomycete’ metabolites;the taxonomic status of positive strains were analyzed by constructing 16S rRNA phylogenetic tree.Results] We cloned and expressed the intact gene of human pancreatic α-amylase successfully;the high-throughput screening model of α-amylase inhibitors was established;nearly 2000 actinomycete’ metabolites were screened,14 α-amylase inhibitor producing strains were obtained finally,and showed taxonomically rich diversity.Conclusion] The α-amylase inhibitor high-throughput screening model had high practical value for developing new hypoglycemic drugs.
%K α-amylase inhibitor
%K Pichia pastoris expression system
%K human pancreatic α-amylase
%K actinomycetes
%K high-throughput screening<br>α-淀粉酶抑制剂
%K 毕赤酵母表达系统
%K 人胰腺α-淀粉酶
%K 放线菌
%K 高通量药筛
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=EEC77E274DE7318A38AABC03DEF631E2&yid=9377ED8094509821&vid=987EDA49D8A7A635&iid=5D311CA918CA9A03&sid=2D52C01434B71375&eid=1AA557EFF1C6B447&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=13