Screening of optimal signal peptide for heterologous xylanase secretion by Bacillus Subtilis
外源木聚糖酶在枯草芽胞杆菌中信号肽筛选

Objective] We searched optimal signal peptide for heterologous and exogenous secretion of xylanase in Bacillus subtilis.Methods] We constructed a screening vector for signal peptides from B.subtilis.The Alkali resistance xylanase gene(xynA) from Bacillus pumilus was chosen as reporter gene and cloned into E.coli and B.subtilis shuttle vector pGJ148 which has maltose-inducible promoter Pglv and spectinomycin resistant gene.24 Sec-type signal peptides(SPs) was amplified from B.Subtilis 1A747 and cloned into...