%0 Journal Article
%T Screening of optimal signal peptide for heterologous xylanase secretion by Bacillus Subtilis<br>外源木聚糖酶在枯草芽胞杆菌中信号肽筛选
%A Xin Fan
%A Mingming Yang
%A Pengtao Cao
%A Wei Zhang
%A Wen Zhang
%A Xiuping Song
%A Yuesheng Gong
%A <br>范鑫
%A 杨明明
%A 曹鹏涛
%A 张伟
%A 张雯
%A 宋秀平
%A 龚月生
%J 微生物学报
%D 2011
%I 
%X Objective] We searched optimal signal peptide for heterologous and exogenous secretion of xylanase in Bacillus subtilis.Methods] We constructed a screening vector for signal peptides from B.subtilis.The Alkali resistance xylanase gene(xynA) from Bacillus pumilus was chosen as reporter gene and cloned into E.coli and B.subtilis shuttle vector pGJ148 which has maltose-inducible promoter Pglv and spectinomycin resistant gene.24 Sec-type signal peptides(SPs) was amplified from B.Subtilis 1A747 and cloned into...
%K xylanase gene
%K singal peptides
%K Bacillus Subtilis<br>关键词:
%K 木聚糖酶基因，信号肽，枯草芽胞杆菌
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=B64E2EF1739A75D569BD0D560CAE0895&yid=9377ED8094509821&vid=987EDA49D8A7A635&iid=DF92D298D3FF1E6E&sid=CA0B5EEC0BAD621A&eid=88B4027FEBE4F5FF&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=0