Heterogenous expression and characterization of cag4 in Helicobacter pylori
异源表达的幽门螺杆菌cag4蛋白有溶菌糖基转移酶活性

Objective]To construct prokaryotic fusion gene expression vector pET-28a-cag pathogenicity island protein 4(cag4),express the recombinant fusion protein cag4 and analyze the enzyme activity of recombinant protein.Methods] We cloned cag4 gene,this encoded lytic transglycosylase in CagPAI of Helicobacter pylori NCTC11637 via PCR method.After TA cloning and restriction enzyme digested confirmed,we constructed prokaryotic expression plasmid pET-28a-cag4 and transformed the plasmid into E.coli BL21(DE3) for he...