%0 Journal Article
%T Heterogenous expression and characterization of cag4 in Helicobacter pylori<br>异源表达的幽门螺杆菌cag4蛋白有溶菌糖基转移酶活性
%A Wenkai Wang
%A Qiao Zhong
%A Liping Xie
%A Shihe Shao
%A <br>王文凯
%A 钟桥
%A 谢立苹
%A 邵世和
%J 微生物学报
%D 2011
%I 
%X Objective]To construct prokaryotic fusion gene expression vector pET-28a-cag pathogenicity island protein 4(cag4),express the recombinant fusion protein cag4 and analyze the enzyme activity of recombinant protein.Methods] We cloned cag4 gene,this encoded lytic transglycosylase in CagPAI of Helicobacter pylori NCTC11637 via PCR method.After TA cloning and restriction enzyme digested confirmed,we constructed prokaryotic expression plasmid pET-28a-cag4 and transformed the plasmid into E.coli BL21(DE3) for he...
%K 关键词：幽门螺杆菌
%K cag4蛋白
%K IV型分泌系统
%K 异源表达
%K 酶谱分析
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=46528DA0F58E18F4711F74DCAEA6239C&yid=9377ED8094509821&vid=987EDA49D8A7A635&iid=94C357A881DFC066&sid=039DCCB9394D9766&eid=9BF3B0483F192149&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=14