Comparison of Six Molecular Methods for Differentiation of Lactococcus lactis subsp. lactis and cremoris
6种区分乳酸乳球菌乳酸亚种和乳酸乳球菌乳脂亚种的分子生物学方法比较

Abstract: Objective] To compare six molecular methods for differentiation of Lactococcus lactis subsp. lactis and cremoris. Methods] Six molecular methods, such as 16S rRNA gene analysis, 16S-23S rRNA Intergenic spacer region polymorphism, Denaturing Gradient Gel Electrophoresis (DGGE), Random Amplified Polymorphic DNA (RAPD), Repetitive Extragenic Palindromic-PCR and Restricted Fragment Length Polymorphisms were used to differentiate the reference strains. Results] Except for 16S rRNA gene analysis and 16S-23S rRNA Intergenic spacer region polymorphism, the other protocols were competent. Conclusion] The methods of DGGE and RAPD were more appropriate for differentiation of Lactococcus lactis subsp. lactis and cremoris.