%0 Journal Article
%T Comparison of Six Molecular Methods for Differentiation of Lactococcus lactis subsp. lactis and cremoris<br>6种区分乳酸乳球菌乳酸亚种和乳酸乳球菌乳脂亚种的分子生物学方法比较
%A Jiachao Zhang
%A Fang Wang
%A Haiyan Xu
%A Jie Yu
%A Qiuhua Bao
%A Wenjun Liu
%A Zhihong Sun
%A Heping Zhang
%A <br>张家超
%A 王芳
%A 徐海燕
%A 于洁
%A 刘文俊
%A 包秋华
%A 孙志宏
%A 张和平
%J 微生物学报
%D 2010
%I 
%X Abstract: Objective] To compare six molecular methods for differentiation of Lactococcus lactis subsp. lactis and cremoris. Methods] Six molecular methods, such as 16S rRNA gene analysis, 16S-23S rRNA Intergenic spacer region polymorphism, Denaturing Gradient Gel Electrophoresis (DGGE), Random Amplified Polymorphic DNA (RAPD), Repetitive Extragenic Palindromic-PCR and Restricted Fragment Length Polymorphisms were used to differentiate the reference strains. Results] Except for 16S rRNA gene analysis and 16S-23S rRNA Intergenic spacer region polymorphism, the other protocols were competent. Conclusion] The methods of DGGE and RAPD were more appropriate for differentiation of Lactococcus lactis subsp. lactis and cremoris.
%K Key words: Lactococcus lactis
%K differentiation
%K molecular methods<br>关键词：乳酸乳球菌
%K 分类鉴定
%K 分子生物学方法
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=1327C40BFD1F4FAE6D14FE2B5076D50B&yid=140ECF96957D60B2&vid=771152D1ADC1C0EB&iid=59906B3B2830C2C5&sid=C134FCA51C2CF9D8&eid=937C5AD88B71B15A&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=0