Screening and breeding high 1,3-propanediol producing strains by genome shuffling
1,3-丙二醇产生茵基因组重排育种

Objective]To improve the tolerance of main metabolites,we used genome shuffling to achieve high 1,3-propanediol producing mutants.Methods]Based on 96 deep-well palates containing prepared ended fed-batch broth as an efficient selection method,genome shuffling has been applied in strain improvement.Results]Five high producers were obtained after genome shuffling(LSG1,LSG2,LSG4,LSG5 and LSG6).During batch fermentation(3 L),the 1,3-propanediol production of the five mutants were improved 17.0%,19.0%,12.9%,2...