%0 Journal Article
%T Screening and breeding high 1,3-propanediol producing strains by genome shuffling<br>1,3-丙二醇产生茵基因组重排育种
%A Shengguo Lu
%A Shuang Li
%A Fang Luo
%A Yangyang Zhu
%A Jun Xiong
%A Qingxiong Meng
%A <br>卢圣国
%A 李霜
%A 罗芳
%A 祝扬扬
%A 熊俊
%A 孟庆雄
%J 微生物学报
%D 2011
%I 
%X Objective]To improve the tolerance of main metabolites,we used genome shuffling to achieve high 1,3-propanediol producing mutants.Methods]Based on 96 deep-well palates containing prepared ended fed-batch broth as an efficient selection method,genome shuffling has been applied in strain improvement.Results]Five high producers were obtained after genome shuffling(LSG1,LSG2,LSG4,LSG5 and LSG6).During batch fermentation(3 L),the 1,3-propanediol production of the five mutants were improved 17.0%,19.0%,12.9%,2...
%K Keywords: 1
%K 3-propanediol
%K Klebsiella pneumoniae
%K genome shuffling<br>关键词：1
%K 3-丙二醇，肺炎克雷伯氏杆菌，基因组重排，原生质体，补料发酵终点液
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=33A5CBD43703012763AACE032CDE5984&yid=9377ED8094509821&vid=987EDA49D8A7A635&iid=E158A972A605785F&sid=FE6B7E9BDCCDBAA6&eid=A33A8FD1432A4C3E&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=14