Screening of high taxol producing fungi by mutagenesis and construction of subtracted cDNA library by suppression subtracted hybridization for differentially expressed genes
高产紫杉醇菌株的诱变选育及其差异表达基因消减cDNA文库的构建

Objective]To screen mutants with high yield of taxol,and construct cDNA subtractive library of obtained mutant and primary strain HD1-3.Methods] The spores of taxol-producing fungus HD1-3 were treated by diethyl sulphate(DES),ultraviolet radiation and diethyl sulphate(UV + DES).cDNA subtractive library of taxol producing fungi from the mRNA of obtained mutant with high yield of taxol tester and HD1-3 driver was constructed by using suppression subtracted hybridization(SSH).Results] The optimal conditions...