%0 Journal Article
%T Screening of high taxol producing fungi by mutagenesis and construction of subtracted cDNA library by suppression subtracted hybridization for differentially expressed genes<br>高产紫杉醇菌株的诱变选育及其差异表达基因消减cDNA文库的构建
%A Kai Zhao
%A Lixin Sun
%A Xuan Wang
%A Xiuliang Li
%A Xin Wang
%A Dongpo Zhou
%A <br>赵凯
%A 孙立新
%A 王旋
%A 李秀凉
%A 王歆
%A 周东坡
%J 微生物学报
%D 2011
%I 
%X Objective]To screen mutants with high yield of taxol,and construct cDNA subtractive library of obtained mutant and primary strain HD1-3.Methods] The spores of taxol-producing fungus HD1-3 were treated by diethyl sulphate(DES),ultraviolet radiation and diethyl sulphate(UV + DES).cDNA subtractive library of taxol producing fungi from the mRNA of obtained mutant with high yield of taxol tester and HD1-3 driver was constructed by using suppression subtracted hybridization(SSH).Results] The optimal conditions...
%K Taxol
%K endophytic fungi
%K mutagenic breeding
%K suppression subtracted hybridization
%K cDNA library<br>紫杉醇
%K 内生真菌
%K 诱变育种
%K 抑制性消减杂交
%K cDNA文库
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=B64E2EF1739A75D5BAAC512181D380F2&yid=9377ED8094509821&vid=987EDA49D8A7A635&iid=DF92D298D3FF1E6E&sid=3019419AF3C0F2FB&eid=3C5A0072CBF0FE42&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=0