Development of Genetic Transformation system of Valsa mali of apple mediated by PEG
PEG 介导的苹果腐烂病菌原生质体转化

Objective] The genetic transformation of Valsa mali var.mali was developed by PEG-mediated protoplasts transformation.Method] It was transformed by PEG-induced fusion of protoplasts.The plasmid pBIG2RHPH2-GFP-GUS carrying hph gene was used and Valsa mali var.mali 03-8 isolate was used as the host strain.Result] At 50 mg/mL driselase+10 mg/mL lysing enzymes concentration,the mycelium of Valsa mali var.mali cultured in YEPD medium for 48 h was hydrolyzed in 10 mL enzymes liquid /0.5 g wet mycelium for 2 h....