%0 Journal Article
%T Development of Genetic Transformation system of Valsa mali of apple mediated by PEG<br>PEG 介导的苹果腐烂病菌原生质体转化
%A Jing Gao
%A Yanbo Li
%A Xiwang Ke
%A Zhensheng Kang
%A Lili Huang
%A <br>高静
%A 李艳波
%A 柯希望
%A 康振生
%A 黄丽丽
%J 微生物学报
%D 2011
%I 
%X Objective] The genetic transformation of Valsa mali var.mali was developed by PEG-mediated protoplasts transformation.Method] It was transformed by PEG-induced fusion of protoplasts.The plasmid pBIG2RHPH2-GFP-GUS carrying hph gene was used and Valsa mali var.mali 03-8 isolate was used as the host strain.Result] At 50 mg/mL driselase+10 mg/mL lysing enzymes concentration,the mycelium of Valsa mali var.mali cultured in YEPD medium for 48 h was hydrolyzed in 10 mL enzymes liquid /0.5 g wet mycelium for 2 h....
%K protoplast
%K transformant
%K hph gene
%K transformation efficiency
%K stability test<br>关键词:
%K 原生质体，转化子，hph
%K 基因，转化效率，稳定性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=D1920AB56F28E37CCCF569AFF30FD418&yid=9377ED8094509821&vid=987EDA49D8A7A635&iid=9CF7A0430CBB2DFD&sid=1805714CB338690A&eid=EB3DE2AA673AE431&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=31