Molecular cloning, gene expression and characterization of purine nucleoside phosphorylase from Pseudoalteromonas sp. XM2107
假交替单胞菌XM2107嘌呤核苷磷酸化酶基因克隆表达、重组蛋白纯化及酶学性质

Objective] Purine nucleoside phosphorylase(PNP,EC 2.4.2.1) is an important enzyme which is applied in nucleoside medication and intermediate biosynthesis.In this paper,we aimed to obtain the PNP gene from cold-adapted marine bacterium Pseudoalteromonas sp.XM2107 and study the characteristics of enzyme for applying in nucleoside medication and intermediate biosynthesis.Methods] Purine nucleoside phosphorylas gene which amplified from the cold-adapted marine bacterium Pseudoalteromonas sp.XM2107 genome by h...