%0 Journal Article
%T Molecular cloning, gene expression and characterization of purine nucleoside phosphorylase from Pseudoalteromonas sp. XM2107<br>假交替单胞菌XM2107嘌呤核苷磷酸化酶基因克隆表达、重组蛋白纯化及酶学性质
%A Guanglu Wang
%A Jungang Xi
%A Xixian Xie
%A Qingyang Xu
%A Ning Chen
%A <br>王光路
%A 夏俊刚
%A 谢希贤
%A 徐庆阳
%A 陈宁
%J 微生物学报
%D 2010
%I 
%X Objective] Purine nucleoside phosphorylase(PNP,EC 2.4.2.1) is an important enzyme which is applied in nucleoside medication and intermediate biosynthesis.In this paper,we aimed to obtain the PNP gene from cold-adapted marine bacterium Pseudoalteromonas sp.XM2107 and study the characteristics of enzyme for applying in nucleoside medication and intermediate biosynthesis.Methods] Purine nucleoside phosphorylas gene which amplified from the cold-adapted marine bacterium Pseudoalteromonas sp.XM2107 genome by h...
%K Keywords: Pseudoalteromonas
%K purine nucleoside phosphorylase
%K enzymatic properties<br>关键词:
%K 假交替单胞菌
%K 嘌呤核苷磷酸化酶
%K 酶学性质
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=7E1AB25FC35A203C781DE8FA48EC32F3&yid=140ECF96957D60B2&vid=771152D1ADC1C0EB&iid=0B39A22176CE99FB&sid=8B59EA573021D671&eid=0DEB7A8A66C33AAD&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=0